Dissecting the Molecular Anatomy of Tissue

Dissecting the Molecular Anatomy of Tissue

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The book provides an updated overview of molecular analysis of human tissues, and the impact this analysis has on diagnosis and prognosis of human diseases. Special emphasis is placed on human cancer and the future directions of the field. Methods of handling clinical tissue samples, including the impact of handling on subsequent molecular analysis, are also discussed. In addition, detailed protocols for molecular analysis of DNA, RNA and protein, with special emphasis on molecular analysis of highly complex human tissue samples containing mixtures of cell populations, are provided.A 1-ll aliquot of each cRNA was analyzed using an Agilent Bioanalyzer 2100 and RNA Nano Chip assay. ... 9.4.3 Troubleshooting Initially, 10 ng of control RNA ( consisting of a high quality RNA source diluted to 10 ng/ll) should be utilized with anbsp;...

Title:Dissecting the Molecular Anatomy of Tissue
Author: Michael R. Emmert-Buck, John W. Gillespie, Rodrigo F. Chuaqui
Publisher:Springer Science & Business Media - 2006-03-30

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